8F Grasshopper

January 27, 2026

Sample information
Picture
Entry by: Harper L
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

large greenish brown grasshopper.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

followed Wolbachia project protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

all controls worked, and bugs that were Wolbachia positive showed that again in the results from DNA sequencing
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
GGTGCATGAGCTGGATAGTAGGAACTTCTATAAGAATAATTATTCGAGCAGAACTAGGTCAACCAGGATCTTTAATTGGAGATGACCAAATCTATAATGTTATTATTACAGCCCACGCATTTGTAATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGATTTGGTAATTGACTTGTACCATTAATAATTGGAGCACCAGATATAGCATTTCCACGAATAAATAATATAAGTTTTTGACTTTTACCACCATCACTAACTCTTTTACTTACCTCATCCATGGTTGATAATGGAGCTGGTACAGGATGAACAGTTTACCCTCCGCTCGCCGGAGCAATTGCACATGGAGGAGCCTCAGTTGATTTAGCCATTTTCTCTCTTCACTTAGCTGGTATTTCATCAATTCTAGGAGCAGTTAATTTTATTACAACAGCAATCAATATACGATCAGAAAGAATAACATTAGACCAAACACCATTATTTGTTTGATCAGTAGTTATTACAGCACTCCTTTTATTATTATCACTTCCTGTACTAGCAGGAGCTATTACAATATTACTAACTGACCGAAACTTAAATACATCATTCTTCGATCCTGCAGGAGGGGGTGACCCAATTCTATATCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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