6I Pavement ant

January 26, 2026

Sample information
Picture
Entry by: Annika K
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found this ant after school on the sidewalk with other ants.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeaGreen
Gel images
Protocol notes

Followed Wolbachia project protocols

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I trust my results because I followed the protocol and compared my specimens’ DNA to a ladder in the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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