Pavement Ant solomon

Sample information

Picture
Entry by: Solomon RB
Location
Collection date 09/05/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Absolutely tiny black ants living in a colony of thousands in dirt and woodchips next a man made pond near a house. Similar ants have been seen making colonies of similar size in sidewalk just around the block. Tiny black ants with large abdomen connected with small nodes. Thorax is larger compared to the abdomen than one of a little black ant, giving me confidence that this is a pavement ant

Putative identification Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium immigrans

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain
Gel images
Protocol notes

I was in class and we did this project and the teacher gave us a “PCR cocktail” that has all of the ingredients for PCR to work but was also colored green. The Gel electrophoresis was done with other students in the same gel and my DNA was in the circles area. I have the arthropod bar but no wolbachia bar

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident because we did all the steps according to plan, all the controls worked well, and I never had any issues while extracting DNA

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tetramorium immigrans was found to be negative for Wolbachia.
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