6N Pavement Ant

January 24, 2026

Sample information
Picture
Entry by: Solomon RB
Location
Collection date 09/05/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Absolutely tiny black ants living in a colony of thousands in dirt and woodchips next a man made pond near a house. Similar ants have been seen making colonies of similar size in sidewalk just around the block. Tiny black ants with large abdomen connected with small nodes. Thorax is larger compared to the abdomen than one of a little black ant, giving me confidence that this is a pavement ant
Putative identification Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium immigrans

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident because we did all the steps according to plan, all the controls worked well, and I never had any issues while extracting DNA
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Tetramorium immigrans was found to be negative for Wolbachia.
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