Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | I found this centipede in the dirt outside of Camp OSRUI in Wisconsin. It was located near a basketball court and several cabins in a grassy area surrounded by trees. It has a long body, numerous legs, orange antennae, and a yellowish hue. |
| Putative identification | Arthropoda Chilopoda Geophilomorpha |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | TBE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level |
Lane 8 |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Geophilomorpha was found to be negative for Wolbachia. |


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