Pillbug 4

January 14, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

It was wet & cloudy, under damp rock, near a path, temp 72 degree F, 8:45 am, near Raritan river.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Standard protocol

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Out of the two times we did the test properly, we’ve gotten wolbachia negative and wolbachia negative. We are unsure of  what caused this.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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