Sample information

The arthropod was found by a tunnel web going into a nearby fence

Very small Potters Grass Spider (Agelenopsis potteri )
Had light brown legs (lightest area of the spider)
Had a black/brown body with a light brown stripe down the center

Arachnida

Methods

Figure 1: Gel electrophoresis showing Arthropod DNA results, after 35 minutes of electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG arthropod sample (old), lane 3 contains KC arthropod sample (old), lane 4 contains a positive arthropod control (old), lane 5 contains a negative arthropod control (old), lane 6 contains RB arthropod sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive arthropod control (new), and lane 11 contains a negative arthropod control (new).

Figure 2: Gel electrophoresis showing Wolbachia DNA results, after 30 minutes after electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG wolbachia sample (old), lane 3 contains KC wolbachia sample (old), lane 4 contains a positive wolbachia control (old), lane 5 contains a negative wolbachia control (old), lane 6 contains RB wolbachia sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive wolbachia control (new), and lane 11 contains a negative wolbachia control (new)

Results

The results showed that there was an error in the Arthropod gel due to poor band results and possible contamination. But the Wolbachia gel showed positive results and the presence of Wolbachia in the DNA.