Arthropod Collection Data – Agelenopsis potteri

February 6, 2026

Sample information
Picture
Entry by: Kayleigh C
Location
Collection date 09/17/2025
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

The arthropod was found by a tunnel web going into a nearby fence

Very small Potters Grass Spider (Agelenopsis potteri )
Had light brown legs (lightest area of the spider)
Had a black/brown body with a light brown stripe down the center

Arachnida
Putative identification Arthropoda Arachnida

Methods
Extraction kit Blood and Tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

 

 

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The results showed that there was an error in the Arthropod gel due to poor band results and possible contamination. But the Wolbachia gel showed positive results and the presence of Wolbachia in the DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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