Arthropod #2 – Termite

Sample information

Picture
Entry by: Kayleigh C
Location
Collection date 10/20/2022
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Small Arthropod

Black, almost shriveled

Was given to the group for a second test

Putative identification Arthropoda Insecta Isoptera

Methods

Extraction kit Qiagen kit for blood and tissues.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Figure 1: Gel electrophoresis showing Arthropod DNA results, after 35 minutes of electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG arthropod sample (old), lane 3 contains KC arthropod sample (old), lane 4 contains a positive arthropod control (old), lane 5 contains a negative arthropod control (old), lane 6 contains RB arthropod sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive arthropod control (new), and lane 11 contains a negative arthropod control (new)

Figure 2: Gel electrophoresis showing Wolbachia DNA results, after 30 minutes after electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG wolbachia sample (old), lane 3 contains KC wolbachia sample (old), lane 4 contains a positive wolbachia control (old), lane 5 contains a negative wolbachia control (old), lane 6 contains RB wolbachia sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive wolbachia control (new), and lane 11 contains a negative wolbachia control (new)

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The arthropod gel was not accurate due to poor joy loading the gel. However the Wolbachia gel was accurate, and the results on the termite was accurate.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isoptera was found to be negative for Wolbachia.
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