Wolbachia Project

January 29, 2026

Sample information
Picture
Entry by: Alyssa U
Location
Collection date 11/04/2025
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

Color from yellow-orange to red, may have 0–19 black spots on the wing covers, black “M” or “W” on white area behind head, size about 7–8 mm long, oval, dome-shaped body, short legs, antennae
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBG
DNA stain SYBR Safe
Gel images
Protocol notes

Standard

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My lab partner and I followed the steps of the procedure correctly for this sample, but the results of the other samples were not correct.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
>Asian Lady Beetle TATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGNGGGTTTTGGAAA TTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCC CTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCT TCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGG TGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGAT CTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGA AACTTAAATTCTTCTTNTTTTGACCCNACCGGTGGGGGAGNNCCAATTTNATACNNACATTTANTTTGNNTTTTNNNNNN NNNNNNNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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