Madison Adams – Harmonia axyridis

January 29, 2026

Sample information
Picture
Entry by: Madison A
Location
Collection date
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

Orangeish red with black spots, Wings under a hard shell, 8mm diameter, circular-shaped shell
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBG
DNA stain SYBR Safe
Gel images
Protocol notes

STANDARD

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

A mistake could have been made when extracting DNA or in the PCR tube because a band didn’t show up
Wolbachia 16S sequence
Arthropod COI sequence
Asian Lady Beetle TATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGNGGGTTTTGGAAA TTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCC CTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCT TCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGG TGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGAT CTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGA AACTTAAATTCTTCTTNTTTTGACCCNACCGGTGGGGGAGNNCCAATTTNATACNNACATTTANTTTGNNTTTTNNNNNN NNNNNNNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
Report Inappropriate Post