Maile Bentz- Harmonia axyridis

January 29, 2026

Sample information
Picture
Entry by: Maile B
Location
Collection date 11/05/2026
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

a small (about 5-6 mm), round beetle that can be red, orange, or yellow with black spots and six legs
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit Qiagen DNEASY
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBG
DNA stain SYBR-SAFE
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My results were not confirmed by my teacher, but my partner and I both agreed our insects did not have the Wolbachia strain.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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