1-KV

February 24, 2026

Sample information
Picture
Entry by: Kamryn_V
Location
Collection date 02/15/2026
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Captured inside of home after specimen had entered through opening in window during winter. Likely seeking warmth and food source. Killed the specimen before collection.
Putative identification Arthropoda Insecta Diptera Muscidae Musca Musca domestica

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010). The specimen was incubated for 53 minutes in a hot water bath at 56 degrees C. The written protocol was followed closely.

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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