Sample information
Picture	Entry by: Eva S
Location
Collection date	03/05/2026
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations	under some rotten, damp tree bark
Putative identification	Arthropoda Insecta Coleoptera Carabidae Pterostichus Pterostichus niger
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Partial abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	agarose gel electrophoresis
Buffer	1X TAE
DNA stain	SYBR-SAFE
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	There are no visible bands in the gel image.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Pterostichus niger was found to be negative for Wolbachia.