Sample information

The spider was collected in Fatima’s home’s backyard.

Methods

DNA Extraction: I crushed the arthropod’s abdomen throughly with some help from my groupmates. I also made sure there was no exoskeleton.
Electrophoresis of both runs:
Lane 1. 1Kb ladder, showed up for both runs.
Lane 2. Western black widow, showed up for CO1 arthropod run, but not for Wolbachia.
Lane 3. Jumping spider, showed up for CO1 arthropod run, but not for Wolbachia.

Lane 4. Positive control, showed up for both runs.

Lane 5. Negative control, barely showed up in CO1 arthropod run.

Lane 6. Positive DNA control, showed up for both runs.

Lane 7: Water, showed in neither run.

Results

The reason why we are confident in our observations is that the water was uncontaminated and there was no positive wolbachia bands on our negative control. Plus, all of the bands showed up for the arthropod run (it’s very faint for well 5, sorry if it’s hard to see). Seeing that there are no errors, we are confident that our arthropod is uninfected.