Sample information

*Found dead
*Discovered on a school sidewalk about 5 feet away from a tree
*No other insects were seen around discovery site
*Found on a cold afternoon in December
*Hemiptera is small in size with black and brown colors
*Long antennae that stick out forward
*The wings are layered on top of each other and flattened out
*Across the wings is a squiggly light brown line
*Legs are folded
*Small head size

Methods

DNA Extraction:
During the DNA extraction I made sure to be very careful. I crushed up the partial abdomen and stayed away from the exoskeleton. There was not trouble when crushing the lead footed bug.

Gel Electrophoresis Lanes:
1. DNA Ladder
2. Hemiptera DNA
3. Araneae DNA
4. Positive Arthropod Control
5. Negative Arthropod Control
6. Positive DNA Control
7. Water

Analysis:
After reviewing my results, all of the controls worked correctly. The positive DNA control also worked on both gels showing that PCR went well. On the arthropod gel, the Hemiptera had a band so we know DNA extraction worked correctly. On the wolbachia gel, there was no band that came up. This will help us conclude that the Hemiptera was not infected with wolbachia.

Results

I am very confident in my results. All the controls showed up in the correct way. A water band did not show in either gel so there is no contamination. The positive DNA control showed in both gels proving that PCR worked correctly. Also, the positive and negative controls worked correctly. Since the Hemiptera showed up in the arthropod gel, we know the DNA extraction worked. Since it showed up in the arthropod, all the controls were correct, and there was no band in the wolbachia gel, I am confident that my results are correct. This means that the Hemiptera is not infected with wolbachia.