Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Auburn University at Montgomery |
| Observations | Very small; bright yellow with a dark bottom tip underneath a piece of wood mostly covered with leaves and soil |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed protocol with no problems. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TTGGTTCTTCTATAAGAATAATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAATACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCTTAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTCAATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAATACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAATCAATTTTATTTCAACAATTTTAAACATACATAATTCTAATATTACCTTAGATAAAATTCCCCTATTAGTTTGATCTATTCTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCTCAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGACCCAATTCTATACCA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR