GM2

March 30, 2026

Sample information
Picture
Entry by: Giovana M
Location
Collection date 03/05/2026
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations
  • Small, elongated insect
  • Dark black body
  • Red/Orange lines along the wings
  • Six legs
  • Long antenna
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  • Samples loaded with loading dye
  • Gen run: 20 mins under blue light

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level
  • No positive/negative controls were included
  • Without controls, PCR success and contamination cannot be confirmed
  • No Wolbachia bands were detected
  • Couldn’t retrieve sequences due to results not coming back
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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