Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Auburn University at Montgomery |
| Observations | It is a millipede that was caught in the AUM forest. |
| Putative identification | Arthropoda Diplopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Gel electrophoresis did not have a DNA sequence, and it was wolbachia negative. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls may have not work due to contamination of my bug DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diplopoda was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR