AX01

April 2, 2026

Sample information
Picture
Entry by: Ashley X
Location
Collection date 03/05/2026
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

Found in AUM forest. It is a western boxelder bug that is black and has a red vertical line on it’s back.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Taq polymerase = GO Taq MM

55 degrees Celsius for both PCR reactions. (Annealing temperature)

 

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level


The controls did work because I did have a band for my arthropod DNA but no bands for wolbochia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Hemiptera was found to be negative for Wolbachia.
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