AL_02

April 2, 2026

Sample information
Picture
Entry by: Arniya L
Location
Collection date 03/05/2026
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

Small red beetle. Has hard shell with block spots. Observed on classmate shirt.
Putative identification Arthropoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

I followed protocol with no problems.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am highly confident in my results because the controls worked as expected. The QS values were above the acceptable threshold, and blast results showed a strong match. Since the sequence aligned well with the known Wolbachia sequences and there were no major issues with the data, I can trust the results.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGANATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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