Sample information
Picture	Entry by: Kendrick Espinal
Location
Collection date	09/11/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations
Putative identification	Arthropoda Insecta Hymenoptera Apidae Bombus
Methods
Extraction kit	Qiagen DNeasy Blood and Tissue Kit.
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	All came back negative
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Bombus was found to be negative for Wolbachia.