Sample information
Picture
Location
Collection date	09/11/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations
Putative identification	Arthropoda Insecta Lepidoptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Im highly confident that it was negative because there weren’t enough bands on the target gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Lepidoptera was found to be negative for Wolbachia.