Sample information

Fly found outside during snowfall. Alive but not moving much.

Methods

We tested three insect samples for the presence of Wolbachia. DNA was extracted from the insect sample and then amplified using PCR. The PCR products were analysed with gel electrophoresis using the MiniOne gel electrophoresis system, TAE buffer and Cyber Green DNA stain.

The Wolbachia DNA control worked and showed the expected positive result. The water control also worked and showed no contamination. The positive and negative controls for animal DNA also gave the expected results.

Overall, the controls show that the experiment worked correctly.

Gel image explanation:

Columns:

1. Fly DNA
2. Mosquito DNA
3. Spider DNA
4. Insect infected with Wolbachia (positive probe)
5. Insect not infected with Wolbachia (negative probe)
6. Wolbachia DNA
7. Water probe
8. DNA-Marker

Results

The fly sample showed a negative result, meaning that no Wolbachia DNA was detected.

The controls worked as expected: the Wolbachia DNA control was positive (column 6) and the animal DNA controls also gave the correct results, with a positiv and a negative result (column 4 and 5). This shows that the DNA extraction, PCR and gel electrophoresis worked properly and that there was no contamination.

Therefore, we can conclude that the tested fly was most likely not infected with Wolbachia.