ROM-JNM

April 18, 2026

Sample information
Picture
Entry by: Jaeden M
Location
Collection date 01/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found under metal pole in GSU Armstrong parking lot using forceps and gloves.

Common names: Grasshoppers,
crickets, locusts, and katydids

Habitat:
Terrestrial/Grasslands/Deserts/
Marshes/ Caves

Diet: Herbivores/Scavengers
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Using past local Wolbachia testing data from Orthoptera specimen collections in 2013-2015, I found that 25% of the specimen tested positive for Wolbachia. With this data from 2013-2015, the numbers of Wolbachia infected Orthoptera specimen likely rose in 11-13 years. Using gel electrophoresis, it was confirmed that Wolbachia Orthoptera specimen have gone up in the past 11-13 years with a positive test from the ROM-JNM specimen sample.
Wolbachia 16S sequence
CGAGACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAA CTTCAtgttctCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTGG GGATTAGCTTAGGCTTGCGCACCTTGCAACCCATTGTAGACACCATTGTA GCACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCC CACCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTACTCAGCATTA CCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCA ACATCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATC CGGCCGAACCGACCCTATCCCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTGGAGCTTGAGCTGGgaTaGTAGGCACATCATTAAGTATTTTAATTCGA ACAGAACTAGGACAACcatgcTATTTAATTGGAGATGATCAAACTTATAA TGTTATCGTAACCGCACATGCATTTATCATAATTTTCTTTATAGTTATAC CTATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTGGGG GCTCCAGATATAGCCTTCCCACGAATAAACAATATAAGATTTTGACTTCT ACCCCCATCATTAACCCTTTTATTAACCAGAAGAATAGTTGAAAATGGTG CAGGAACAGGATGAACAGTATACCCACCTCTATCAACAGGAATCGCTCAC GCGGGAGCATCCGTCGATTTAGCTATCTTCTCACTACATTTAGCAGGAAT TTCCTCAATTCTAGGGGCTGTAAATTTTATCACTACCATAATTAATATAC GAGCTCCAGGAATATCACTAGACCAAACACCATTATTTGTTTGAGCAGTC GGAATTACAGCTCTTCTATTATTATTATCCTTACCAGTTTTAGCTGGTGC TATTACAATATTGCTTACAGATCGAAATTTAAATACATCATTTTTTGATC CAGCGGGAGGAGGGGATCCAATCTTATATCAACATCTATTTTGATTTTTT GGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be postive for Wolbachia.
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