ROM-AJR_Diptera

April 13, 2026

Sample information
Picture
Location
Collection date 01/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

DNA was sent for sequencing and results align with gel electrophoresis results
Wolbachia 16S sequence
Arthropod COI sequence
ATCTCTAAGAATTTTAATTCGAGCTGAATTAGGTCATccatgAGCATTAA TTGGAGATGACCAAATTTATAACGTAATTGTTACCGCACATGCTTTTATT ATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTG ATTAGTTCCATTAATATTGGGGGCCCCAGATATAGCATTCCCACGAATAA ATAATATAAGATTTTGACTACTGCCCCCTGCTCTTTCTTTATTATTAGTG AGAAGAATGGTTGAAAACGGAGCTGGAACAGGTTGAACTGTTTACCCACC TCTTTCTGCTGGAATTGCTCATGGAGGGGCTTCAGTAGATTTAGCAATTT TTTCATTACATCTAGCCGGAATTTCTTCAATTTTAGGAGCTGTAAATTTT ATTACAACTGTAATTAATATACGATCAACGGGTATTACATTAGACCGAAT ACCTTTATTTGTTTGATCAGTTGTAATTACTGCTTTATTACTTTTATTAT CATTACCAGTACTAGCTGGAGCTATTACTATATTATTAACAGATCGAAAT TTAAATACCTCATTTTTTGACCCGGCAGGAGGAGGGGATCCTATTTTATA TCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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