April 13, 2026

Sample information
Picture
Entry by: Sidney V
Location
Collection date 01/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • mandibles
  • three sections of body
  • antennae
  • 6 legs, lacks prolegs
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The entire process of DNA extraction to the gel electrophoresis went smoothly, so there would be no error that would result in a falsely negative result.
Wolbachia 16S sequence
Arthropod COI sequence
TaatTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTgt tattCTCTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACA CGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGAT TTGGAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTAT CCTCGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCT TTTACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTA TCTACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGAT CTATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGC TATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTA TAGATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTT CTTTTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAAC TGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATC CCATTCTATACCAACATTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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