JTS-CHI – Orthoptera

April 15, 2026

Sample information
Picture
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Caught with hands
  • Found in tall grass
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No Wolbachia sequence and no visible line during Gel Electrophoresis
Wolbachia 16S sequence
Arthropod COI sequence
AGCAGGTATAGTAGGAACATCAATAAGAATAATTATTCGAGCTGAACTTG GTCAGccatgatcTCTTATTGGAGATGATCAAATTTATAATGTAATTATT ACAGCACACGCATTTGTAATAATTTTTTTTATAGTTATACCAATTATAAT TGGAGGATTCGGTAATTGATTAGTACCTCTTATAATTGGAGCACCAGACA TAGCCTTTCCTCGAATAAATAATATAAGATTTTGACTATTACCTCCGTCA TTAACCCTTTTATTAATATCCTCTATAGTCGATAATGGAGCTGGTACAGG ATGAACAGTTTACCCACCGTTAGCTAGAACTATTGCCCACAGAGGAGGAG CAGTTGATTTAGCAATTTTTTCTCTACACTTAGCAGGTGTCTCATCTATT CTAGGAGCAGTAAATTTTATTACAACTGCAATTAATATACGATCAGAAAG AATAACATTAGATCAATCACCATTATTTGTTTGATCAGTAGCTATTACAG CATTATTGTTATTACTCTCATTACCAGTTCTAGCAGGTGCCATCACTATA CTATTAACAGACCGAAATCTTAATACATCATTCTTTGATCCAGCTGGAGG AGGTGATCCAATTTTATATCAACACTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
Report Inappropriate Post