CHI-TDL Diptera

April 15, 2026

Sample information
Picture
Entry by: Tristen L
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Forest wetlands
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All control results show as expected
Wolbachia 16S sequence
Arthropod COI sequence
TGGATAGTAGGAACATCCTTAAGTATTTTAATTCGAGCTGAATTAGGACA TccatgagCACTAATTGGAGATGACCAAATTTATAACGTAATTGTTACTG CTCATGCATTTATTATAATTTTCTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCAGATATAGC CTTTCCTCGAATAAACAATATAAGTTTTTGATTATTGCCTCCTGCTTTAA CCCTACTTCTAGCAAGTAGCATAGTAGAAAACGGAGCTGGTACAGGATGA ACTGTTTACCCTCCTCTATCTTCTAATATTGCTCACGGAGGTGCTTCTGT AGATTTAGCTATTTTCTCCTTACACTTAGCTGGAATTTCCTCAATTTTAG GAGCCGTAAATTTTATTACTACTGTAATTAATATACGATCTACAGGAATT ACTTTTGACCGAATACCTTTATTTGTTTGATCAGTAGTAATTACAGCTTT ATTACTTTTATTATCTTTACCTGTTTTAGCTGGAGCTATTACAATATTAT TAACAGATCGAAATTTAAATACTTCATTTTTCGACCCTGCAGGAGGAGGA GATCCTATTCTATACCAACATTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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