Oncopeltus fasciatus

April 21, 2026

Sample information
Picture
Entry by: Sarah G.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My controls worked and my PCR left me with good quality DNA. My sequences came back with good quality and were able to 100% identify my insect and the Wolbachia my insect had.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Oncopeltus fasciatus was found to be postive for Wolbachia.
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