Formica fusca

April 29, 2026

Sample information
Picture
Entry by: Dhuha F
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica Formica fusca

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am moderately confident in the results because the DNA bands observed in the gel did not match the expected band pattern for Wolbachia. Since the Wolbachia-specific band was not present, this suggests that the sample is likely not infected with Wolbachia. However, the presence of amplified DNA still indicates that the organism is most likely an arthropod, as the general arthropod primers successfully produced a band. My confidence is not high because there is still a possibility of experimental error, such as issues with PCR amplification or gel electrophoresis, which could affect the accuracy of the results.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TTGGATCTTCTATAAGTATAATTATTCGATTAGAATTAGGTTCTTCAAATTCATTAATTAATAATGATCAAATTTATAAT TCTTTAGTAACTAATCATGGCTTTATTATAATCTTTTTTATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCT AATTCCTTTAATATTAGGGTCACCAGATATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTTTTACCTCCTTCCA TCACTCTATTACTTTTAAGAAACTTTATTAATGATGGTACTGGAACTGGATGAACTATCTACCCTCCTTTAGCTTCAAAT ATTTTTCATAATGGACCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCCGGTATATCATCTATTTTAGGAGCTAT TAATTTTATTTCAACAATTATTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCCCTACTTGTATGATCTATCT TAATCACTGCAATTTTACTTTTATTATCCCTTCCCGTTTTAGCAGGGGCTATTACAATATTATTAACTGACCGAAACTTA AATACTTCATTTTTTGACCCTTCAGGAGGGGGGGACCCAATTCTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica fusca was found to be negative for Wolbachia.
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