DS Wolbachia Project

May 6, 2026

Sample information
Picture
Entry by: DivyaS
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

“Collected by Fall 2025 students”
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low because I have no Wolbachia band present in my arthropod.
Wolbachia 16S sequence Download FASTA   
GTATTCNANCCGTGGCGTGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAA CTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGNCGGNANCNTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTG TAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGT CCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCT GACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
GTAACAGCCCATGCATTTATTATAATTTTCTTTATAGTAATACCAATTATAATTGGTGGCTTCGGAAACTGACTAGTACC TTTAATAATTGGAGCACCTGATATAGCATTTCCACGAATAAATAATATAAGATTCTGATTATTACCACCTTCATTAACCC TTCTTTTATCTAGAAGAATTACAGAAAGAGGAGCTGGAACAGGATGAACTGTTTATCCTCCTCTATCTAATAGAATTTTT CACAGAGGAGCATCTGTAGATATGGCTATTTTTTCTCTACATTTAGCAGGAATTTCATCAATTCTGGGTGCAATTAATTT TATTTCAACAATTTCAATAAGANTNGAATTATTCGAATTGAATTAGGTCAACCAGGTATATTTATTGGAAATGACCAAATTTATAATGTAATT ATTAACATACGACCAACAGGTATGTCTTTAGAAAAAACCCCTTTATTTGTATGGTCAGTAGGAATTA CAGCTCTATTGCTTCTATTATCCTTACCTGTATTAGCAGGAGCAATCACAATACTATTAACTGATCGAAACTTTAATACC TCATTCTTTGACCCTACAGGAGGAGGAGACCCAATTTTATATCAACATCTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
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