Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | black with red rim and eyes. 6 legs. antennae. visible lines on abdomen |
| Putative identification | Arthropoda Insecta Hemiptera Rhopalidae Boisea Boisea trivittata |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We used a DNA extraction protocol based on the insect adaptation of New England Biolabs Monarch Spin gDNA extraction kit (product T3010) The specimen was incubated for 45 minutes in a hot water bath at 56 degrees Our first gel image was taken 3/24/2026 and was run at 125 volts for 19 mins and 20 secs. Used DNA ladder: New England Biolabs 1kb Plus DNA Ladder for Safe Stains (product #N0559S) Our SECOND PCR reaction was set up on 3/31 used the same Taq polymerase as the first PCR reaction, and: was a single PCR reaction that used the Wolbachia F and Wolbachia R primers, and an annealing temperature of 55 degrees celsius. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Moderately confident because my controls all came out with the expected result but some doubt because additional PCR could reveal Wolbachia presence. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Boisea trivittata was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR