Benedictine University Spring 2026 (Harmonia axyridis)

April 26, 2026

Sample information
Picture
Entry by: Lizzy W.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Gel had a weak Wolbachia band, but a 100.00% ID match in BLAST. Some of the controls did not have the proper arthropod or Wolbachia band reducing their reliability in determining whether or not my sample was positive for Wolbachia. Additionally, there was an error in loading my specimen DNA into the gel, which could explain the weak arthropod/Wolbachia band.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Harmonia axyridis was found to be postive for Wolbachia.
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