Formica schaufussi

April 26, 2026

Sample information
Picture
Entry by: Caroline P
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

During gel electrophoresis, my arthropod presented a strong Wolbachia gel band but had a 99.00%-99.99% BLAST match.
Wolbachia 16S sequence Download AB1
CCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
AATTCATTAATTAATAATGATCAAATTTATAATTCTTTAGTAACTAATCATGCCTTTATTATAATCTTTTTTATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATATTAGGGTCACCAGATATAGCTTATCCTCGTATAAATAATATAAGATTTTGACTTTTACCTCCTTCCATCACTCTATTACTTTTAAGAAACTTTATTAATGATGGTACTGGAACTGGATGAACTATCTACCCTCCTTTAGCTTCAAATATTTTTCATAATGGACCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCCGGTATATCATCTATTTTAGGAGCTATTAATTTTATTTCAACAATTATTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCCCTACTTGTATGATCTATCTTAATCACTGCAATTTTACTTTTATTATCCCTTCCCGTTTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formica was found to be postive for Wolbachia.
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