Caelifera

April 27, 2026

Sample information
Picture
Entry by: Yusra S
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collection done by Fall 2025 students
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus sanguinipes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Proper lab safety occurred and DNA was found using the Nanodrop system.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is lower because the DNA isolation had values for the 260/280 of 1.71 which would suggest a protein concentration and a 260/230 of 0.61 which is likely a salt contaminant.
Wolbachia 16S sequence Download FASTA    Download AB1
CGGTTCGCCCGGCGGGTGACAGAAGCGAAAACTATTCACCGTGGCATGCTGATCACGATTCCTCGCGATTCAATCCATGA CTCGATCGCAGAGTACAATCCGAACCAGACGTCTTTAGGGATTAGCTTAGGCTTGCGCACCTCGCAGCCCATTGTAGACA CCATCGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGAGACTTGACATCATCCCCACCTTCCTCCAGCTTATCACTG GCAGTTTCCTTAAAGTACTCAGCATTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA TCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCCGGCCGAACCGACCCTATCCCTTAAGGTATGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTCGGGACTAGTAGGAACTTCTATAAGAATAATTATTCGAGCAGAACTAGGACAACCAGGATCTCTAATTGGAGATGAC CAAATCTATAATGTAATTATTACAGCCCACGCATTTGTAATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGATT TGGTAATTGACTTGTACCATTAATAATTGGAGCACCAGATATAGCTTTTCCACGAATAAATAATATAAGTTTTTGACTTT TACCACCATCACTAACCCTTTTACTTACCTCATCTATGGTTGATAATGGAGCTGGTACAGGATGAACAGTTTACCCCCCA CTCGCTGGAGCAATTGCACATGGTGGAGCGTCAGTTGATTTAGCCATTTTCTCTCTTCACTTAGCTGGTGTTTCATCAAT TCTAGGGGCAGTCAATTTTATTACAACAGCAATTAATATAAGATCAGAAAGAATAACACTAGACCAAACACCATTATTTG TTTGATCAGTAGCTATTACAGCACTCCTTTTATTATTATCACTTCCTGTATTAGCAGGAGCTATTACAATATTATTAACT GATCGAAATTTAAATACATCATTCTTTGACCCTGCGGGAGGAGGTGATCCAATTTTATATCAACATTTATTTTGATTTTT TGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus sanguinipes was found to be negative for Wolbachia.
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