April 27, 2026

Sample information
Picture
Entry by: Zak S
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Insecta Lepidoptera Pieridae Pieris Pieris rapae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls worked because the expected results were observed (the positive control showed amplification and the negative control showed no amplification), which means the PCR process functioned correctly without contamination. Because my sample showed 100% presence of Wolbachia and the controls behaved properly, I can trust that my results are accurate and reliable.
Wolbachia 16S sequence Download FASTA    Download AB1
CCCGTTTTTGTGTTTTCACAGATATGTTGCTGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAGAAAGCAACCCTCATCCTTAGTTACCATCAAATAATGCTGGGGACTTTAAGGAAACTGCTATTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACGATGGGCTGCAGAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCACCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTATTAATTCATCTAAGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>uknowninscentz TACCGGAGCGCGACGGCCGCTTGACCACCTCCGAGGGTCGAAAAAATGTATCTCAGTTCCGGTCCGTCAAAAGCATAGTGTAGCCCCTGCCAATACCGGCAAAGAAAGCAACAATAAAAGAGCAGTAACGGCTACGGATCATACAAACAACGGTATTCGTTCCAGCTTAAGGCCCGATGGGCGCATATTAATCACCGTTGTGATAAAATTAATTGCCCCCAAAATTGATGAAATCCCTGCCAAATGCAATGAAAAAATACTCAAATCTACCGAAGCCCCTGCGTGGGCGATGGCCCCCGACAAAGGGGGGTAAACTGTCCAACCTGTACCAGCTCCTCTATCTACCATACTCCCAGAAAGCAAAAGCATAAGCGAAGGGGGTAGTAACCAAAAACTCATGTTGTTTATTCGGGGAAAGGCCATATCTGGGGCTCTCAACATCAAAGGTACCAGTCAATTCCCAAAACCTCCAATCATAATAGGCATTACCATAAAAAAAATCATTACAAAAGCATGGGCCGTAACAATTACATTATAAATTTGATCATCTCCGATTAAAGCTCCAGGTTGGCCCAATTCTGCCCGAATCAACAAGCTCAATGAAGTCCCCACTATTCCTGATCAAGCCCCAAATACAAAATATAAAGTTCCAATATCTTTATGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pieris rapae was found to be postive for Wolbachia.
Report Inappropriate Post