Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students. |
| Putative identification | Arthropoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | No DNA was found at all in our experiment. |
| Wolbachia 16S sequence |
>42-C_WP2_Wo_R.ab1 (119 bp) CGCGGCGGGTGACGTCATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCAT GCAACACTTGTGTAAAATCCGGCCGAACCGACCCTATCC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
>DNA sample arthropod.scf (632 bp) TGGGAGCTGTAGGAACTAGAATAATTATTCGAGCAGAACTAGGACAACCAGGATCTCTAATTGGAGATGACCAAATCTAT AATGTAATTATTACAGCCCACGCATTTGTAATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGATTTGGTAATTG ACTTGTACCATTAATAATTGGAGCACCAGATATAGCTTTTCCACGAATAAATAATATAAGTTTTTGACTTTTACCACCAT CACTAACCCTTTTACTTACCTCATCTATGGTTGATAATGGAGCTGGTACAGGATGAACAGTTTACCCCCCACTCGCTGGA GCAATTGCACATGGTGGAGCGTCAGTTGATTTAGCCATTTTCTCTCTTCACTTAGCTGGTGTTTCATCAATTCTAGGGGC AGTCAATTTTATTACAACAGCAATTAATATACGATCAGAAAGAATAACACTAGACCAAACACCATTATTTGTTTGATCAG TAGCTATTACAGCACTCCTTTTATTATTATCACTTCCTGTATTAGCAGGAGCTATTACAATATTATTAACTGATCGAAAT TTAAATACATCATTCTTTGACCCTGCGGGAGGAGGTGATCCAATTTTATATCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR