Sample information
Picture	Entry by: leslieD
Location
Collection date	09/03/2025
Captive / Cultivated?
Group	Benedictine University
Observations
Putative identification	Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Gel had 2 bands indicating Arthropod DNA and presence of second band wolbachia presence.
Wolbachia 16S sequence	Download FASTA
Arthropod COI sequence	Download FASTA
Summary	The Armadillidium vulgare was found to be postive for Wolbachia.