Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2024 students |
| Putative identification | Arthropoda Insecta Hymenoptera Apidae Bombus Bombus impatiens |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Sanger Sequencing revealed low quality DNA, and the BLAST results showed no match. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
GGGCGGCGGGATATTGCGGAAAAAGGATCCTTTTATTGTTTTGGCAACAACTCCGGTCTTCATATTTTTTTTTTTAATAGGCACACCAAACCCAACCGGGAGAAGGGGGTTTTATTTTAATACCTTCAACTGCAGGACTCGCGGTCTTCCCTTCCATAATGTAAATAATATAAAGGTCGGATAATCCCGCCCATGATGATTTAAATAAATCCCCACCCCGAAAGGAACACACTGGGGAGGAAACGGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Bombus impatiens was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR