Velarifictorus micado

April 28, 2026

Sample information
Picture
Entry by: Faryal I.
Location
Collection date 09/02/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Caught by Fall 2024 students
Putative identification Arthropoda Insecta Orthoptera Gryllidae Velarifictorus Velarifictorus micado

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am confident because I had a 100% ID BLAST match, but did not have Wolbachia in my sample.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TGGGCTTGGGAATAGTCGGCACATCACTAAGTATTTTAATTCGAACGGAATTAGGACAACCAGGGTATCTTATTGGAGAT GATCAAACATATAATGTAATTGTTACTGCACACGCCTTTATTATAATTTTCTTCATAGTAATACCAATTATGATTGGAGG ATTCGGAAATTGATTAGTTCCCTTAATATTAGGAGCACCCGATATAGCTTTCCCCCGAATAAATAATATAAGTTTTTGAT TATTACCCCCTTCATTAACTCTCCTATTAACCAGAAGAATAGTTGAAAATGGAGCAGGTACAGGATGAACAGTTTATCCA CCCTTGTCAACAGGAATTGCCCATGCAGGAGCATCAGTTGATTTAGCAATTTTCTCATTACATCTCGCCGGAATCTCCTC CATTTTAGGTGCTGTAAATTTTATTACTACCATAATTAATATACGAGCTCCTAGAATGTCTTTAGATCAAACACCCCTAT TTGTGTGAGCAGTAGGAATTACAGCACTATTATTATTATTATCATTACCTGTACTAGCAGGAGCTATTACTATACTATTA ACTGATCGAAACTTAAATACCTCATTTTTTGATCCCGCAGGAGGAGGAGATCCAATCCTATATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Velarifictorus micado was found to be negative for Wolbachia.
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