Spider

July 16, 2021

Sample information
Picture
Photos by: Paul P and Braydon T, Alex L
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Found on stairs outside in the shade.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Used the abdomen

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Can’t really see
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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