Parajulidae Millipdes

July 16, 2021

Sample information
Picture
Photos by: Nora G
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Arthropod was found in mulch and dirt under a tree. It has a dark back with a tan belly. The legs are red.
Putative identification Arthropoda Myriapoda Diplopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I used the whole arthropod and it is located in the seventh well.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diplopoda was found to be postive for Wolbachia.
Report Inappropriate Post