|Captive / Cultivated?||Wild-caught|
|Group||Norwich Free Academy|
Arthropod was found in mulch and dirt under a tree. It has a dark back with a tan belly. The legs are red.
|Putative identification||Myriapoda Diplopoda|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
I used the whole arthropod and it is located in the seventh well.
|Explanation of confidence level||
This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Diplopoda was found to be postive for Wolbachia.|