Pollenia rudis

April 28, 2026

Sample information
Picture
Entry by: Hayden L.
Location
Collection date 09/06/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Insecta Diptera Polleniidae Pollenia Pollenia rudis

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

see gel
Wolbachia 16S sequence Download FASTA    Download AB1
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATAATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGCACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pollenia rudis was found to be postive for Wolbachia.
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