Formica spp

April 30, 2026

Sample information
Picture
Entry by: Suleman Z
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Some DNA came out of the tube when pestling. Had high salt concentration suggesting contamination.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Had 100% ID match for my insect.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Formicidae was found to be negative for Wolbachia.
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