Citrus Mealybug

April 28, 2026

Sample information
Picture
Entry by: Allison C
Location
Collection date
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

No Wolbachia results. Standard CO1 primers did not yield DNA amplification, so used Pco1F and LepR1 primers. These yielded CO1 results, but did not test for Wolbachia.
Putative identification Arthropoda Insecta Hemiptera Pseudococcidae Planococcus Planococcus citri

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
TTGGATTTTGATCTGGATTAATAGGTTTATCAATAAGATTTATTATTCGAATTGAACTAATAAATTTAAATAATAATTTTAATAACAATATAATTTATTATATAATAATTACTATTCATGCTTTTATTATAATTTTCTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTTTTACCATTAATATTAATATCATCAGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATAAATATAATATTATCTAATAATATTAATACAGGTTGAACACTTTACCCCCCTTTAATTAATCAAAATTTTATTACATTAAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTTTTATTATCAATAATAATAATTTTTTTTTAAATAATATTACTTTATATATTTGATCTATTATTATTACAACTATTTTATTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTTTAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTTTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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