Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2025 students. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Qiagen DNeasy Blood and Tissue Kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | After conducting a nano-drop results showed a high salt concentration. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Gel band was dark and large indicating a high presence of Wolbachia DNA. After BLASTing the results showed a 99.59% for percent identity and an E-Value of 8e-822. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR