May 2, 2026

Sample information
Picture
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students.
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Low confidence level because the gels did not turn out usable, however my BLAST results were 99.5%.
Wolbachia 16S sequence Download FASTA    Download AB1
GGCTTGGCAGGGCAGTGTGTACAGACCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACT TCATGTACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTGGCTTAGTCTCGCGACTTTGCAGCCC ATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCA GTTTATCACTAGCAGTTTCCTTAAAGTCCCCAGCATTATCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGAC TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCT TCAAATAGGTATGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CCATATCTTTATATTTGGATATGATCTGGAATAGTAGGTTCATCAATAAGATGAATTATTCGAATTGAATTAGGTCAACC AGGTATATTTATTGGAAATGACCAAATTTATAATGTAATTGTAACAGCCCATGCATTTATTATAATTTTCTTTATAGTAA TACCAATTATAATTGGTGGCTTCGGAAACTGACTAGTACCTTTAATAATTGGAGCACCTGATATAGCATTTCCACGAATA AATAATATAAGATTCTGATTATTACCACCTTCATTAACCCTTCTTTTATCTAGAAGAATTACAGAAAGAGGAGCTGGAAC AGGATGAACTGTTTATCCTCCTCTATCTAATAGAATTTTTCACAGAGGAGCATCTGTAGATATGGCTATTTTTTCTCTAC ATTTAGCAGGAATTTCATCAATTCTGGGTGCAATTAATTTTATTTCAACAATTATTAACATACGACCAACAGGTATGTCT TTAGAAAAAACCCCTTTATTTGTATGGTCAGTAGGAATTACAGCTCTATTGCTTCTATTATCCTTACCTGTATTAGCAGG AGCAATCACAATACTATTAACTGATCGAAACTTTAATACCTCATTCTTTGACCCTACAGGAGGAGGAGACCCAATTTTAT ATCAACATCTATTTTGATTTTTTGGCTCGTTTCAGTAGCCCCCCCCAAACCTATCCCGGGGGCTTTGGGCGGCACCGGGG GGTCGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
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