Ant 2 BASD

May 4, 2026

Sample information
Picture
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

Sample was caught from a yeast trap in a wooded area of western Pennsylvania during late summer, about 64°, fair conditions.
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system BioRad
Buffer TAE
DNA stain UView
Gel images
Protocol notes

DNA Extraction: My whole insect was included; I did not dissect it.
Gel electrophoresis lanes for CO1 arthropod PCR:
– Lane 1: Ladder
– Lane 2: Insect sample #1 (Ant 2)
– Lane 3: Insect sample #2 (Spotted Lanternfly)
– Lane 4: Positive fruit fly
– Lane 5: Negative fruit fly
– Lane 6: Positive DNA
– Lane 7: Negative water
Analysis: Gels ran as expected. Lane 6 was faint in Wolbachia gel, but still visible.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence is high because all of my controls were present, ruling out a high chance of contamination. I did not have any issues with the protocol, so I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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