Wasp BASD

May 4, 2026

Sample information
Picture
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

I found this sample on a car in the staff lot of our high school. It was late summer, early fall with a temperature of 64 degrees while conditions were fair with no rain or clouds. Out of all the bugs on the car, the wasp stood out the most.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 0.25 TAE
DNA stain UView
Gel images
Protocol notes

DNA Extraction: We made sure to dissect the abdomen of the wasp and left out the exoskeleton.
Gel Electrophoresis:
1- ladder

2- insect 1

3- insect 2

4- + fruit fly

5- -fruit fly

6- + DNA

7- – water

Analysis: my control lanes were all correct and showed up. So I can rely on the data that there was no Wolbachia in the wasp.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked and showed up how they should have. Everything aligned with the protocol therefore I am confident that my sample doesn’t have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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