Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Beaver Area School District |
| Observations | I took swabs from my friends car, during late summer. The specimen was disassembled when we found it. This was when the weather was around 59 degrees F outside. |
| Putative identification | Arthropoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane1: ladder lane2: moth lane3: gnat lane4: + fruit fly lane 5: – fruit fly lane6: + DNA lane 7: – water |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia gel The positive controls showed up as expected. I didn’t have any issues with the protocol either. I’m confident my arthropod isn’t infected with wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR