BASD gnat

May 4, 2026

Sample information
Picture
Entry by: Brittany S
Location
Approximate location: 40.70, -80.30
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

We took swabs from the student parking lot off of cars. The temperature outside was around 50 degrees F.
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

1: ladder

2: moth

3: gnat

4: positive fruit fly

5: negative fruit fly

6: positive DNA

7: negative DNA

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Gel:


I am confident that my arthropod did not contain Wolbachia. All lanes results were as expected and nothing out of the ordinary. Our positive controls also showed up as expected.

 
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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